APP (KPI-DOMAIN); RB X-100UL

Code: AB5302 D2-231

Application

Western blot: 1:250 - 1:1,000

Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.

Immunocytochemistry: On Cos7 and...


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Application

Western blot: 1:250 - 1:1,000

Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.

Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.

Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.

Optimal working dilutions must be determined by end user.

Reduction /Alkylation protocol:

To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.

Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.

Immunogen

APP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Specificity

Amyloid Precursor Protein (APP), KPI Domain.

antibody formserum
antibody product typeprimary antibodies
biological sourcerabbit
clonepolyclonal
Gene Informationhuman ... APP(351)
manufacturer/tradenameChemicon®
NCBI accession no.NM_000484.2, NM_201413.1, NM_201414.1
Quality Level100
shipped indry ice
species reactivitymonkey, mouse, bovine, human
technique(s)immunocytochemistry: suitable, western blot: suitable, immunoprecipitation (IP): suitable, immunohistochemistry: suitable
UniProt accession no.P05067
This product has met the following criteria to qualify for the following awards:



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