Application
Western blot: 1:250 - 1:1,000
Immunoprecipitation: Requires reductions and alkylation of disulfide bonds.
Immunocytochemistry: On Cos7 and NteraZ cell lines, requires reduction and alkylation of disulphide bonds.
Immunohistochemistry: Works on formaldehyde fixed, paraffin embedded tissue, requires reduction and alkylation of disulphide bonds or microwaving.
Optimal working dilutions must be determined by end user.
Reduction /Alkylation protocol:
To reduce incubate the slide (Blot) in TBS (PBS) + 50mM 2-mercaptoethanol at 37°C for 3 h in the dark. Wash twice with TBS, then incubate in TBS (PBS) + 20 mg/ ml of Iodoacetic acid for 30 min at room temperature. Wash twice with TBS (PBS), block and process as usual.
Anti-Amyloid Precursor Protein Antibody, KPI Domain is an antibody against Amyloid Precursor Protein for use in IP, WB, IC, IH.
Immunogen
APP.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Specificity
Amyloid Precursor Protein (APP), KPI Domain.
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